The Zhaobishan gold deposit, as one of the most important gold deposits in northwest China, is located at the convergence area of the Siberian, Kazakhstanian and Tarim paleoplates. The gold mineralization occurred within the Zhaobishan biotite granitoid stock as quartz vein and veinlet group. The systematic field investigations showed that the gold mineralization was spatially and temporally related to the Hercynian granitoid magmatism. The oxygen and hydrogen isotopic analyses on quartz separates from the gold bearing quartz veins and the granitoid revealed that the ore fluid for the deposit was mainly derived from a mixed source of Hercynian igneous fluid and Paleozoic meteoric water. The lead isotope data on pyrite, chalcopyrite and gold ore samples from the Zhaobishan area constituted a mixing line cutting the Pb growth curves of upper crust, orogen, lower crust and mantle. Two chalcopyrite, one pyrite and two gold ore samples from the gold bearing quartz veins fell between the areas of the Hercynian biotite granite samples. The result of lead isotope analyses indicated the Hercynian granitoid being initial lead source for the gold bearing quartz veins. Both geological evidences and isotope data demonstrated that the lead, gold and other metal elements of the gold bearing vein system mainly came from the Hercynian granitoids which was probably derived from the re melting of previously formed supracrustal rocks during Paleozoic orogeny. Meanwhile, the genetic model and mineral exploration criteria of the Zhaobishan gold deposit could also be used during the comprehensive evaluation of the concealed gold deposits in the Beishan region.