The Fuyu oil layer of Sanzhao depression in northern Songliao Basin developed a set of low permeable tight sandstone reservoirs formed in river to shallow-water delta environments. Proven reserves was preserved in conventional reservoir rocks, and lithology reservoir was the main reservoir type. While the remaining exploration targets will be tight reservoir with porosity less than 10% and permeability less than 1×10-3 μm2. Based on analysis of tight oil accumulation in Sanzhao depression of northern Songliao Basin, three unconventional resource assessment methods, which are volumetric method of small cells, resource abundance analogy and EUR analogy, have been used to evaluate tight oil in Fuyu oil layer of Sanzhao depression in this paper. Hydrocarbon generation quantity of lacustrine source rocks in the 1st member of Qingshankou Formation has also been estimated based on the genetic resources assessment. Therefore, a serial of key parameters, such as geological resource, hydrocarbon migration and accumulation coefficient, recovery rate, resource abundance of tight oil in this area can be calculated, which represent key parameters of a type of tight oil that below source rocks in Songliao Basin. This study can also be used for reference when the same kind of tight oil evaluation is carried out in other areas.