Diverse, abundant and well-preserved radiolarians were discovered from the chert of middle and upper members of the Gufeng Formation(Loer Permian)in Hushan area, Naming(Jiangsu Province). Fourteen genera and twenty seven species are recognized and described. Of these, one genus(Quadrilobata)and one species(Quadrilobata ephippiomorpha)are new. These radiolarians are composed of three basic types of microfossils, namely, the albaillellids, the spherical polycystine and the stauraxon polycystine. Pseudoalbaillella fusiformis, Hegleria mammilla and Ruzhencevispongus uralicus are dominant in the Gufeng Formation. Based on the distribution pattern of the radiolarians in the Gufeng Formation, two assemblage zones, namely, the Pseudoalbaillella fusiformis-P. longtanensis Assemblage(Ass.)in the lower part and the Ruzhencevispongus uralicus Ass. in the upper part of studied Formation of Hushan, Nanjing(Jiangsu Province)have been proposed, Biostratigraphically, the first Ass. is correlated with the Pseudoalbaillella scalprata-P. fusifo rmis Ass. from Gufeng Formation in Longtan, Naming and the Pseudoalbaillella.longtanensis Ass. of Southwest Japan. The second Ass. is considered as equivalent to the Pseudoalbaillella globosa Ass. of Southwest Japan and the Ruzhencevispongus uralicus Ass. of Southern Urals, USSR. Therefore, it is considered that the two zones fall into the middle and late Maokouan stage respectively, corresponding to middle and late Guadalupian.